Coding

Part:BBa_K5207004

Designed by: Ruyi Shi   Group: iGEM24_Shanghai-BioX   (2024-09-30)
Revision as of 07:49, 30 September 2024 by Kexinfei (Talk | contribs)

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CYP76AH3

It belongs to the CYP76AH subfamily of cytochrome P450 oxygenases. It is a hybrid catalytic P450 that catalyzes the hydroxylation of ferredoxin at the C11 position and the carbonylation of tanshinone at the C7 position, generating 11-hydroxyferredoxin, lupulin, and 11-hydroxylupulin.The carbonylation function at the C7 position allows for the extension of the tanshinone biosynthesis pathway into an additional branched chain, resulting in the formation of a complex catalytic network. We inserted it into pYTK001 by method above.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 695
    Illegal SapI.rc site found at 1445


Usage and Biology

Figure 1. Validation plot of target gene gel electrophoresis

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