Coding

Part:BBa_K5207003

Designed by: Ruyi Shi   Group: iGEM24_Shanghai-BioX   (2024-09-30)
Revision as of 07:46, 30 September 2024 by Kexinfei (Talk | contribs)

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CYP76AH1

It is from the CYP76AH subfamily of cytochrome P450 oxygenases. It is a ferredoxin synthase that catalyzes the C12 hydroxylation of miltiradiene. We inserted it into pYTK001 by method above.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1417
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 695
    Illegal SapI.rc site found at 1445


Usage and Biology

Figure 1. Validation plot of target gene gel electrophoresis

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