Coding
Part:BBa_K5195010
Designed by: Alice Finkelstein Group: iGEM24_Stanford (2024-09-29)
Revision as of 07:46, 30 September 2024 by Alicefinkelstein (Talk | contribs)
Description: Base pairs: 1425
Usage and Biology: In order to be able to quantify how much DUX4-DBD protein would be produced in cells upon transfection, we constructed a coding sequence consisting of an mNeonGreen (mNG) sequence followed by a Peptide 2A (P2A) linker upstream of the DUX4-DBD. The P2A linker causes ribosomal “skipping” during translation, and this effectively separates the mNG and DUX4-DBD proteins. In designing the coding sequence in this way, we enabled quantification of DUX4-DBD without directly attaching another large protein to it.
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