Part:BBa_K5226052

321(C+cysNC) backbone

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BamHI site found at 2528
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NgoMIV site found at 2981
  Illegal NgoMIV site found at 3866
  Illegal NgoMIV site found at 4977
  Illegal NgoMIV site found at 5101
  Illegal AgeI site found at 2037
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI site found at 2103
  Illegal SapI.rc site found at 2171
  

Introduction

One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to obtain ,Halomonas strains that can produce tyrian purple. We chose to introduce four enzymes that is either necessary or beneficial to the production of tyrian purple.

Usage and Biology

This part is a native ribosomal binding site of stth, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts to facilitate the expression of Stth.