Composite

Part:BBa_K5117020:Design

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-08-31)
Revision as of 07:04, 30 September 2024 by Jesa98 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


BsRBS-BhrPET


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 594
    Illegal AgeI site found at 639
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,

addition of a signal peptide SPaprE for secretion in Bacillus subtilis,

addition of start codon (ATG) and stop codon (TAA)

Source

Bacterium HR29