Part:BBa_K5291010

hok/mok

The gene encoding a kind of toxic protein that can lead to bacteria death, whose mRNA will be repressed by sok antisense RNA.

Usage and Biology

The gene hok/mok from hok/sok system can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system. And sok, the gene also in hok/sok system, can encode a kind of antisense RNA to specifically bind to hok mRNA, repressing the expression of toxin.


Fig.1 The schema of hok/mok (the gene on the right)



Fig.2 The mechanism of hok/sok system

Fig.3 The AGE figure of hok/sok

For convenience, we set hok/mok and sok as a whole and didn't prepare both of them separately. You can refer to the part BBa_K5291037 to get to know about the function of hok/mok.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]