Coding
hok

Part:BBa_K5291010

Designed by: Rui Chen   Group: iGEM24_BNUZH-China   (2024-08-10)
Revision as of 04:34, 30 September 2024 by Kortybones (Talk | contribs)


hok/mok

The gene encoding a kind of toxic protein that can lead to bacteria death, whose mRNA will be repressed by sok antisense RNA.


Usage and Biology

The gene hok/mok from hok/sok system can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system. And sok, the gene also in hok/sok system, can encode a kind of antisense RNA to specifically bind to hok mRNA, repressing the expression of toxin.


Fig.1 The schema of hok/mok (the gene on the right)


Fig.2 The mechanism of hok/sok system

Fig.3 The AGE figure of hok/sok

For convenience, we set hok/mok and sok as a whole and didn't prepare both of them separately.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None