DNA

Part:BBa_K5291039:Design

Designed by: Haosheng Lin   Group: iGEM24_BNUZH-China   (2024-09-30)
Revision as of 04:29, 30 September 2024 by Kendy (Talk | contribs)

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PQJ: cumate-inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Leveraging the PQJ promoter's responsiveness to cumate, coupled with cumate's established environmental stability and non-toxic profile, we can strategically deploy the PQJ promoter to activate the suicide system in engineered bacteria, achieving precise control over bacterial self-termination.


Source

This Promoter was derived from the literature: A Synthetic Cumate-Inducible Promoter for Graded and Homogenous Gene Expression in Pseudomonas aeruginosa

References

Klotz A, Kaczmarczyk A, Jenal U. A Synthetic Cumate-Inducible Promoter for Graded and Homogenous Gene Expression in Pseudomonas aeruginosa. Appl Environ Microbiol. 2023 Jun 28;89(6):e0021123. doi: 10.1128/aem.00211-23. Epub 2023 May 18. PMID: 37199671; PMCID: PMC10304978.