Part:BBa_K5531006

pET-Dual-N-His-TEV

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NotI site found at 150
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BglII site found at 305
  Illegal BamHI site found at 119
  Illegal XhoI site found at 354
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NgoMIV site found at 324
  Illegal NgoMIV site found at 671
  Illegal NgoMIV site found at 5341
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1256
  

BBa_K5531006 (pET-Dual-N-His-TEV)

Profile

Name: pET-Dual-N-His-TEV
Base Pairs: 5413 bp
Origin: Escherichia coli; synthesized
Properties: A plasmid for efficient co-expression of two target proteins in Escherichia coli, wherein one of the two target proteins expressed can be His-tagged, and a TEV protease cleavage site follows the His-tag. The plasmid contains two polyclonal sites (MCS), each preceded by a T7 promoter/lac operon and a ribosome binding site (RBS), which can efficiently initiate the expression of the target proteins induced by IPTG.

Fig. 1. Plasmid map of pET-Dual-N-His-TEV