Composite

Part:BBa_K5226087

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-30)
Revision as of 03:54, 30 September 2024 by Registry (Talk | contribs)

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PR/PRM -tnaA -fmo

One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to obtain Halomonas strains that can produce tyrian purple. We chose to introduce four enzymes that is either necessary or beneficial to the production of tyrian purple. There were stth,fre,tnaA and fmo. Because both Stth and TnaA can utilize tryptophan, and tryptophan has a stronger preference for TnaA than for Stth, we introduced the thermalsensitive bio-switch that we built for Halomonas TD to separate the expression of the two enzymes to increase yield.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1960
    Illegal PstI site found at 724
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1960
    Illegal PstI site found at 724
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1960
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1960
    Illegal PstI site found at 724
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1960
    Illegal PstI site found at 724
    Illegal NgoMIV site found at 1912
    Illegal AgeI site found at 1347
    Illegal AgeI site found at 2056
    Illegal AgeI site found at 2386
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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