Composite

Part:BBa_K5520004

Designed by: Ruyi Shi   Group: iGEM24_Ulink-SZ   (2024-09-29)
Revision as of 03:49, 30 September 2024 by Kexinfei (Talk | contribs)


pT7-LacO-His-CDA

This part contains BBa_K2406020, BBa_B0034, BBa_K5520002 and BBa_K731721. BBa_B0034 contains a natural ribosome binding site (RBS) to facilitate the expression of downstream genes. By inducing efficient expression of the CDA protein through the addition of IPTG, the protein can subsequently be purified using the His tag for further enzyme activity tests and product polymerization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 149
    Illegal NheI site found at 897
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 182
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 641


[edit]
Categories
Parameters
None