Coding

Part:BBa_K5335025

Designed by: Yuanpu Zhang   Group: iGEM24_HZAU-China   (2024-09-29)
Revision as of 02:33, 30 September 2024 by Zhangyuanpu (Talk | contribs)


SpyCatcher-VDAL-CPPs (R9-Tag) 

    To enhance plant immunity, a novel fusion protein, SpyCatcher-VDAL-CPPs, was designed. This construct incorporates a SpyCatcher tag【1】 for conjugation, VDAL for immune activation【2】, and the R9 cell-penetrating peptide for intracellular delivery【3】. Functional studies demonstrated the capacity of this fusion protein to penetrate plant cell membranes and induce an ETI response. Molecular characterization and DAB staining confirmed the successful construction and immunogenic activity of the fusion protein.

The design was verified as shown in Figure 1. 无标题文档


Figure 1. Experimental circuit design diagram

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None