Composite

Part:BBa_K5324013:Design

Designed by: Sebastian Nolte   Group: iGEM24_Alma   (2024-09-26)
Revision as of 02:06, 30 September 2024 by Seabass (Talk | contribs)

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SPINK6 Secretion Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SPINK6 is codon optimized for E. coli.


Source

SPINK6 originated from Homo sapiens.

References

1 - Nih.gov. (2024). SPINK6 serine peptidase inhibitor Kazal type 6 [Homo sapiens (human)] - Gene - NCBI. [online] Available at: https://www.ncbi.nlm.nih.gov/gene/404203.
2 - Thomas, S., Holland, I.B. and Schmitt, L. (2014) ‘The type 1 secretion pathway — the hemolysin system and beyond’, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1843(8), pp. 1629–1641. doi:10.1016/j.bbamcr.2013.09.017. ‌