Composite

Part:BBa_K5324012:Design

Designed by: Sebastian Nolte   Group: iGEM24_Alma   (2024-09-26)
Revision as of 02:01, 30 September 2024 by Seabass (Talk | contribs)


SPINK8 Secretion Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SPINK8 is codon optimized for E. coli. Bsal and Bsml restriction cut site were removed with mutations.


Source

SPINK8 originated from Homo sapiens.

References

1 - Spink8 serine peptidase inhibitor Kazal Type 8 (putative) [homo sapiens (human)] - gene - NCBI National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=646424.