Composite

Part:BBa_K5324011:Design

Designed by: Sebastian Nolte   Group: iGEM24_Alma   (2024-09-26)
Revision as of 01:47, 30 September 2024 by Seabass (Talk | contribs) (→‎References)


Aprotinin Secretion Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Aprotinin sequence was codon optimized for expression in E.coli.


Source

Aprotinin originated from Bos taurus.

References

1 - Vedvick, T.S. (1993) ‘DNA for expression of aprotinin in methylotrophic yeast cells’.
2 - Moitra, V. et al. (2009) ‘What is the best means of preventing perioperative renal injury?’, Evidence-Based Practice of Anesthesiology, pp. 226–239. doi:10.1016/b978-1-4160-5996-7.00034-1.
3- Thomas, S., Holland, I.B. and Schmitt, L. (2014) ‘The type 1 secretion pathway — the hemolysin system and beyond’, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1843(8), pp. 1629–1641. doi:10.1016/j.bbamcr.2013.09.017.