Composite
Part:BBa_K5324011:Design
Designed by: Sebastian Nolte Group: iGEM24_Alma (2024-09-26)
Aprotinin Secretion Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Aprotinin sequence was codon optimized for expression in E.coli.
Source
Aprotinin originated from Bos taurus.
References
1 - Vedvick, T.S. (1993) ‘DNA for expression of aprotinin in methylotrophic yeast cells’. 2 - Moitra, V. et al. (2009) ‘What is the best means of preventing perioperative renal injury?’, Evidence-Based Practice of Anesthesiology, pp. 226–239. doi:10.1016/b978-1-4160-5996-7.00034-1.