Coding

Part:BBa_K196004

Designed by: Laetitia Warny   Group: iGEM09_ULB-Brussels   (2009-08-13)
Revision as of 22:52, 21 October 2009 by Aline (Talk | contribs)

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HfsG + HfsH proteins from Caulobacter crescentus

Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically. Besides the bacterium is able to synthesize a strong glue. This glue is mainly composed of polysaccharide. Different proteins are required in the holdfast synthesis, export and attachment in C.crescentus (for more details, please refer to the "introduction" section). In our project, we wanted to implement the glue production in Escherichia coli. As E. coli possesses homolog proteins, we decid to insert the hfsG and hfsH genes in a plasmid. HfsG[1]is a glycosyltransferase and HfsH [2] is a carbohydrate esterase. Here is the biobrick composed og the hfsG and hfsH genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 480
    Illegal AgeI site found at 379
    Illegal AgeI site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None