Coding
Part:BBa_K5439006:Design
Designed by: Diego Cota Barocio, Ruben Valenzuela Sanchez Group: iGEM24_TecMonterreyGDL (2024-09-30)
FRET-based system for the detection of ibuprofen
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2306
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1177
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2174
Illegal BsaI.rc site found at 2959
Design Notes
NcoI and XhoI restriction sites were added at the 5' and 3' ends. There are no scars between genes.
Source
BBa_K1159302, BBa_K5439005, BBa_K1907000