Part:BBa_K5034201:Design

pYYDT plasmid

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 11
  Illegal PstI site found at 3787
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 11
  Illegal PstI site found at 3787
  Illegal NotI site found at 2828
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 11
  Illegal BglII site found at 3574
  Illegal XhoI site found at 4985
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 11
  Illegal PstI site found at 3787
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 11
  Illegal PstI site found at 3787
  Illegal NgoMIV site found at 556
  Illegal NgoMIV site found at 4238
  Illegal NgoMIV site found at 4521
  Illegal AgeI site found at 396
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 3150
  Illegal SapI.rc site found at 4087
  Illegal SapI.rc site found at 4297
  

Design Notes

We synthesized the fragment using a chemical synthesis method.

Source

From Shewanella oneidensis

References

Yang, Y., Wu, Y., Hu, Y., Cao, Y., Poh, C. L., Cao, B., & Song, H. (2015). Engineering Electrode-Attached Microbial Consortia for High-Performance Xylose-Fed Microbial Fuel Cell. ACS Catalysis, 5(11), 6937-6945. https://doi.org/10.1021/acscatal.5b01733