Coding

Part:BBa_K5324000:Design

Designed by: Sebastian Nolte   Group: iGEM24_Alma   (2024-09-22)
Revision as of 22:09, 29 September 2024 by Seabass (Talk | contribs) (→‎References)

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Aprotinin (bovine pancreatic trypsin inhibitor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was codon optimized for expression in E.coli. Was designed due to current part BBa_K1456006having an excess base pair causing a frame shift along with encoding for more than just aprotinin. This was made to have the simplified protein without additional modification beyond aprotinin.

Source

Genomic sequence obtained from literature. Originated from Bos taurus.

References

1 - Vedvick, T.S. (1993) ‘DNA for expression of aprotinin in methylotrophic yeast cells’.
2 - Moitra, V. et al. (2009) ‘What is the best means of preventing perioperative renal injury?’, Evidence-Based Practice of Anesthesiology, pp. 226–239. doi:10.1016/b978-1-4160-5996-7.00034-1.