Coding

Part:BBa_K5324002:Design

Designed by: Sebastian Nolte   Group: iGEM24_Alma   (2024-09-29)
Revision as of 21:54, 29 September 2024 by Seabass (Talk | contribs) (→‎References)


Destructin-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 589
    Illegal NgoMIV site found at 694
    Illegal NgoMIV site found at 832
    Illegal NgoMIV site found at 1054
    Illegal AgeI site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was codon optimized for expression in E.coli. Restriction cut sites were avoided for BsaI, BsmI, and SapI.


Source

Genomic sequence obtained from GeneBank. Originated from P. destructans.

References

Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112 RecName: Full=subtilisin-like protease 1; AltName: Full=destructin-2; National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/939319657.