Composite

Part:BBa_K5089006:Design

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)
Revision as of 20:31, 29 September 2024 by Prefont6 (Talk | contribs) (→‎References)

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CLE-CsgA-LCI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 226
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This expression system is codon optimized for expression in E.coli.


Source

CLE is a cutinase from Cryptococcus sp. strain S-2, previously shown to cleave carbon-chain ester substrates [1]. LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene, polypropylene and polylactic acid [2]. CsgA is a protein component of E.coli biofilms, which can be used as an anchor to surface display proteins [3].

References

1. Masaki, K., Kamini, N. R, Ikeda, H., and Iefuji, H. 2005. Cutinase-Like Enzyme from the Yeast Cryptococcus sp. Strain S-2 Hydrolyzes Polylactic Acid and Other Biodegradable Plastics. Appl. and Environ. Microbiol., 71(11), 7548–7550.https://doi.org/10.1128/AEM.71.11.7548-7550.2005

2. Lu, Y., Hintzen, K.-W., Kurkina, T., Ji, Y., and Schwaneberg, U. 2023. Directed Evolution of Material Binding Peptide for Polylactic Acid-specific Degradation in Mixed Plastic Wastes. ACS Catalysis, 13(19), 12746–12754. https://doi.org/10.1021/acscatal.3c02142

3. Fei Li, Luona Ye, Longyu Zhang, Xiaoyan Li, Xiaoxiao Liu, Jiarui Zhu, Huanhuan Li, Huimin Pang, Yunjun Yan, Li Xu, Min Yang, Jinyong Yan, Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive, Materials Today Bio, Volume 14, 2022.