Coding
Part:BBa_K5035007:Design
Designed by: Lucas Garcia Group: iGEM24_IEA (2024-09-29)
O68252 is a Tetrachloroethene reductive dehalogenase produced by Sulfurospirillum multivorans (Dehalospirillum multivorans). O68252 catalyzes the reductive dechlorination of tetrachloroethene (PCE) to trichloroethene (TCE) and of trichloroethene to cis-1,2-dichloroethene (DCE). O68252 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Similar to A6RdhA, O68252 requires iron, sulfur, and norpseudovitamin B12 as cofactors. Because of O68252's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities.
Design Notes
A hexahistidine tag for nickel column purification was added to O68252.