Coding

Part:BBa_K5035007

Designed by: Lucas Garcia   Group: iGEM24_IEA   (2024-09-29)
Revision as of 18:36, 29 September 2024 by Registry (Talk | contribs)

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O68252 Tetrachloroethene reductive dehalogenase

O68252 is a Tetrachloroethene reductive dehalogenase produced by Sulfurospirillum multivorans (Dehalospirillum multivorans). O68252 catalyzes the reductive dechlorination of tetrachloroethene (PCE) to trichloroethene (TCE) and of trichloroethene to cis-1,2-dichloroethene (DCE). O68252 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Similar to A6RdhA, O68252 requires iron, sulfur, and norpseudovitamin B12 as cofactors. Because of O68252's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1024
    Illegal PstI site found at 1216
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1024
    Illegal NheI site found at 994
    Illegal PstI site found at 1216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1024
    Illegal BamHI site found at 543
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1024
    Illegal PstI site found at 1216
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1024
    Illegal PstI site found at 1216
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1117


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Categories
Parameters
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