Part:BBa_K5237015
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This part can be used for the display of nanobodies on the surface of E.coli. Salema, V. et al, (2013) showed efficient presentation of nanobodies on the surface of E.coli K-12 cells by fusing them to the β domain of Intimin. The part comes from the pNeae2 plasmid (addgene #168300) and encodes an N-terminal signal peptide for Sec-dependent translocation into the periplasm, a LysM domain that interacts with the peptidoglycan and provides anchoring, and a β-barrel that inserts into the outer membrane. The C-terminus is exposed to the extracellular milieu and contains a myc tag (EQKLISEED) that can be used for purification or detection of full-length protein.
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Functional Parameters
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