Coding

Part:BBa_K5416031

Designed by: Jixiao Wu   Group: iGEM24_Imperial-College   (2024-09-29)
Revision as of 18:16, 29 September 2024 by Registry (Talk | contribs)

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PhaC-HRT2trunc

PhaC-HRT2trunc is a fusion protein combining 1. the N-terminus hydrophobic domain of poly-hydroxyalkanoate (PHA) synthase enzyme (PhaC) from C. necator; 2. the turncated HRT2 rubber synthase enzyme (HRT2trunc); and 3. a bridging peptide designed to rigidify the entire structure and positioning the HRT2trunc to the correct orientation. This enzyme is designed to produce rubber granules through a mechanism inspired by the foramtion of PHA carboxysomes inside prokaryotes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None