Regulatory
P_GSK3B
Part:BBa_K5088002
Designed by: Dascha Khalfine Group: iGEM24_Marburg (2024-07-30)
Glycogen synthase kinase 3 - Promoter+5'UTR from T. kok-saghyz
Contents
Background
x
Motivation
Use Cases
Lack of Endogenous Regulatory Parts
Political Context
Plant Synthetic Biology
Gene Structure in Plants
Cis-Regulatory Elements
Design and Characterization
Identification & Design Strategy
Genetic Context of the Part
Measurement
Dual Fluorescence Reporter Assay
Choice of Reporter Genes
Transient Transformation
Leaf Infiltration
Protoplast
Measurement Setup
Results
The Dandelion Toolbox
Overview
Dandelion Handbook
References
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1596
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1596
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 456
Illegal BglII site found at 1518 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1596
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1596
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/eukaryote/plants/other
origin | Taraxacum kok-saghyz |