Coding

Part:BBa_K5035006:Design

Designed by: Lucas Garcia   Group: iGEM24_IEA   (2024-09-29)
Revision as of 18:00, 29 September 2024 by Registry (Talk | contribs)

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A0A7C3HHU1 4Fe-4S dicluster domain-containing protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 68
    Illegal PstI site found at 134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 68
    Illegal PstI site found at 134
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 68
    Illegal PstI site found at 134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 68
    Illegal PstI site found at 134
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A hexahistidine tag for nickel column purification was added to A0A7C3HHU1.


Source

ATGCATCATCATCATCATCATCGCTTCTTTGCTATCGCTATTTACCTGTTCCTGGCTGTGGTTGCGACTGCAGCGAGCATCGTATCTCTGCGTGAAGGCGAATCCCGTGCTTTCCGTCTGAGCGCTCTGCTGTCTGCAGTCGCAGTGGCATGGGCAGGTGTACTGTGGGAAGTTGCGGTATCTGCGCCGTGGCTGGCAACCTGTTCCACCGTTCTGGCCGCTGTTCTGATTACTGCCGCGCTGCTGCCTTACGACCCGGTACGTCGTGCTCTGCGTACTAGCGCACCTCCTTCCAAAAACGTGGACGAACGCGACGTGATCTTTGCGCGCCGTCGTCTGGTAAGCGGCACCACCCGTTTCGAAGCCTACTACAGCGAACATCCGGAAAAACGCGCAGTTGATGACCGTTGGCGTGCTCGTCCGGGCCTGCTGAGCACTGACGCGGCATTTTATCAGCCGCTGTGGTTCGCTGCGGCTGACGCAAGCTTCGACACCATTGAAGCTCTGCACCCTCACGTAGACGGTCCGGTCGAAAACAACCGTGTTCCGCTGGATGCTGTCGAAGCTACCCGCTTTGTGAAAGACTGGCTGAAACACTCTGGTGCGCACTCTGTTGGTGTTACTGCGCTGCGTGATTACCACCTGTATACTCATCGTGGTCGCCAAGAGCCGTACGGTGAACCGATTCGTAAACGTCACCGTTGGGCCATTGCATTCACGGTAGAGATGTCTCACGAACTGCTGCGCTTCGCACCGCTGGCACCGACGGTTCGTGAATCCGCAGACCAATACCTGCGTGCGGGTGCGATGGCTGTAAAACTGGCTTGGATGATCCGCAAGCTGGGTTACCCGGCTCGTGCTCATGTGGACGGCAACTACGAAGTGATTTGCCCGCTGGTGGCGCGTGACGCAGGCCTGGGCGAGATCGGCCGTATGGGTCTGCTGATGACCCCGCGCCTGGGTCCGCGCGTTCGTATTGCTGTTGTAACCACGGACCTGCCGCTGGTTCCGGATGCAGCACAGCCGGACGCCGCGGTTATTGACGCGTGTCTGCACTGCCGTAAGTGCGCCGCTAATTGCCCGGCCCAGGCAATCCCGTTCGGTCCGCGTGTAGATGAACAGGGCGTTCTGCGCTGGCGTATCGACGCGGAAGCATGTTTCACCTACTGGAGCACCGTTGGCACGGACTGTGCGCGTTGCGTGCGTGTTTGCCCGTTCTCCCATCCTGACAACCTACTCTGGCGTCGATGA

References