Regulatory
P_RPL28
Part:BBa_K5088001
Designed by: Dascha Khalfine Group: iGEM24_Marburg (2024-07-30)
Large subunit ribosomal protein L28e - Promoter+5'UTR from T. kok-saghyz
Contents
Background
Motivation
Use Cases
Lack of Endogenous Regulatory Parts
Political Context
Plant Synthetic Biology
Gene Structure in Plants
Cis-Regulatory Elements
Design and Characterization
Identification & Design Strategy
Genetic Context of the Part
Measurement
Dual Fluorescence Reporter Assay
Choice of Reporter Genes
Transient Transformation
Leaf Infiltration
Protoplast
Measurement Setup
Results
The Dandelion Toolbox
Overview
Dandelion Handbook
References
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 510
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 510
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 510
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 510
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 510
Illegal AgeI site found at 1496 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/plants/other
origin | Taraxacum kok-saghyz |