Coding
katB

Part:BBa_K5108002:Design

Designed by: La Breton   Group: iGEM24_Toulouse-INSA-UPS   (2024-08-28)
Revision as of 16:05, 29 September 2024 by Yohannquivet (Talk | contribs)

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Catalase from Pseudomonas fluorescens SBW25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 917
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 148
    Illegal NgoMIV site found at 322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 723
    Illegal SapI.rc site found at 1240


Design Notes

All BioBrick parts used for this part are compatible with the RFC10 Biobrick standard. Fusion of basic parts was performed by In-Fusion Assembly (Takara, France).

Source

Isolated from Pseudomonas fluorescens SBW25. Primers for genomic amplification:
FWD: 5’-ggccgcggccgcgcgaattcaaaaaaaccagaggaaaccctgatg-3’
REV: 5’-cacgacgcggccgcaagctttcagtcagtcagtttttcagcca-3’

References

Uniprot C3K2E2