Composite

Part:BBa_K5246043

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-25)
Revision as of 15:58, 29 September 2024 by Sarpilo (Talk | contribs) (→‎Introduction)


Caulobacter crescentus CB2/CB2A HfsE-HfsJ-HfsG-HfsH-HfsK-HfsL Polysaccharide tetrad assembly

Introduction

Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.

This is the complete holdfast tetrad assembly system. Parts of this composite can be found:BBa_K5246044 and BBa_K5246045.

This part was used in Vilnius-Lithuania iGEM 2024 project "Synhesion" https://2024.igem.wiki/vilnius-lithuania/.

Usage and Biology

TBA

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3690
    Illegal XbaI site found at 4450
    Illegal PstI site found at 4539
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3690
    Illegal PstI site found at 4539
    Illegal NotI site found at 619
    Illegal NotI site found at 3444
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3690
    Illegal BglII site found at 2280
    Illegal BglII site found at 4453
    Illegal BamHI site found at 2428
    Illegal XhoI site found at 2647
    Illegal XhoI site found at 5268
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3690
    Illegal XbaI site found at 4450
    Illegal PstI site found at 4539
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3690
    Illegal XbaI site found at 4450
    Illegal PstI site found at 4539
    Illegal NgoMIV site found at 758
    Illegal NgoMIV site found at 1389
    Illegal NgoMIV site found at 2001
    Illegal NgoMIV site found at 3099
    Illegal NgoMIV site found at 3748
    Illegal NgoMIV site found at 3772
    Illegal NgoMIV site found at 3776
    Illegal AgeI site found at 1088
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental characterization

References

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Categories
Parameters
None