Coding

Part:BBa_K5301001

Designed by: Jiaxin Guan   Group: iGEM24_BNU-China   (2024-09-21)
Revision as of 14:59, 29 September 2024 by Guanjiaxin (Talk | contribs)


SdyCatcher can achieve covalent binding of proteins through Tag-Catcher interaction.

SdyCatcher is a fibronectin-binding protein. It can cooperate with SdyTag to achieve covalent binding of proteins through Tag-Catcher interaction. SdyCatcher and SdyTag can also be used alongside SpyTag-SpyCatcher partners, to demonstrate kinetically controlled directed protein ligation, domain specific protein circularization and macromolecular assembly [1].

Usage and Biology

We used this part to connect two MSP segments..

Characterization

We used SDS-PAGE to test whether this part worked.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
    Illegal AgeI site found at 58
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 55
    Illegal BsaI.rc site found at 256


  1. ↑ Tan, L.L., S.S. Hoon and F.T. Wong, Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. PLOS ONE, 2016. 11(10): p. e0165074.
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Categories
Parameters
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