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Part:BBa_K5382140:Experience

Designed by: yuyanyan chen   Group: iGEM24_HUBU-4-CHN   (2024-09-26)
Revision as of 14:43, 29 September 2024 by Chenyuyanyan (Talk | contribs) (Applications of BBa_K5382140)


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Applications of BBa_K5382140

Extrudable outer membrane vesicles containing Cas9 RNP were delivered into HeLa cells, and the gene-editing activity of prepared OMV-Cas9 RNP was compared with commercial liposomal transfection reagents by T7E1 assay (FIG. 1).

gene-editing1.png
Figure 1.Verification experiment of genome editing activity of Hela cells.

The results of cutting the target gene showed that the prepared OMV-Cas9 RNP had similar genome editing activity to the commercial liposome transfection reagent, both of which were about 40%.

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