Coding
crnA

Part:BBa_K5108004:Design

Designed by: Léa Breton   Group: iGEM24_Toulouse-INSA-UPS   (2024-08-28)
Revision as of 14:22, 29 September 2024 by Leabreton (Talk | contribs)

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Creatinine amidohydrolase form Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199
    Illegal BsaI.rc site found at 547


Sources

The codon-optimized nucleotidi sequence from Pseudomonas putida adapted to Pseudomonas putida was obtained using the Codon Optimizer Tool from Integrated DNA Technologies. This step permits optimize codon usage, lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis.

References

  • UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry
  • Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151