Part:BBa_K5477003

pMEL1 - constitutive core promoter in Saccharomyces cerevisiae

pMEL1 encodes for secreted alpha-galactosidase is an enzyme required for the catabolic breakdown of melibiose into glucose and galactose. This process is regulated by several GAL genes. The enzyme plays a crucial role in carbohydrate metabolism, facilitating the utilization of melibiose as a carbon source (1) (2) (3).

In our system, pMEL1 was used to drive the expression of our reporter module with NanoLuc | BBa_K5477030. This reporter module is combined with the AhR receptor module and therefore comprises our biosensor systems | BBa_K5477041 and | BBa_K5477042 .

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Mortimer RK, Contopoulou CR, King JS. Genetic and physical maps of Saccharomyces cerevisiae, Edition 11. Yeast. 1992 Oct;8(10):817-902. doi: 10.1002/yea.320081002. PMID: 1413997.

2. Naumov G, Turakainen H, Naumova E, Aho S, Korhola M. A new family of polymorphic genes in Saccharomyces cerevisiae: alpha-galactosidase genes MEL1-MEL7. Mol Gen Genet. 1990 Oct;224(1):119-28. doi: 10.1007/BF00259458. PMID: 1980522.

3. Sumner-Smith M, Bozzato RP, Skipper N, Davies RW, Hopper JE. Analysis of the inducible MEL1 gene of Saccharomyces carlsbergensis and its secreted product, alpha-galactosidase (melibiase). Gene. 1985;36(3):333-40. doi: 10.1016/0378-1119(85)90188-x. PMID: 3000884.