Coding

Part:BBa_K5387000

Designed by: iGEM24_Linkoping   Group: iGEM24_Linkoping   (2024-08-20)
Revision as of 13:28, 29 September 2024 by Feliciawedlund (Talk | contribs)

ALOX12B

Usage and Biology

ALOX12B is a gene found on human chromosome 17 and encodes for the enzyme 12R-LOX. The enzyme is an example of an arachidonate lipoxygenase, which is a family of enzymes that has arachidonic acid as a substrate. The enzymes will add an oxygen molecule to the substrate producing a fatty acid hydroperoxide, which later on acts as an important signaling molecule. For the case of 12R-LOX, 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE) is produced when adding an oxygen molecule. 12R-HPETE is then transformed into a signaling molecule important for lipid formation in the epidermis, which serves a necessary role for preventing skin dehydration [1].

The gene codes for one of the enzymes used for iGEM24_Linkoping substitution therapy for ichthyosis.

Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1789
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 722
    Illegal BamHI site found at 2070
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1747
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 454

References

[1] Krieg P, Fürstenberger G. The role of lipoxygenases in epidermis. Biochim Biophys Acta. 2014 Mar;1841(3):390-400. doi: 10.1016/j.bbalip.2013.08.005. Epub 2013 Aug 16. Erratum in: Biochim Biophys Acta. 2014 Dec;1841(12):1767. PMID: 23954555.

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Categories
Parameters
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