Part:BBa_K5382160

InaK-linker-4z domain_protein domain that have the ability to bind to lgG

InaK is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of E. coli cells as an anchor moda, so as to enable surface display.
Linker here is a short peptide used to connect the Inak and z domain.
z domain refers to a synthetic protein domain that has the ability to bind to the Fc region of immunoglobulin G (IgG). In our project, z domains are used to construct extracellular vesicles (EVs) that can display antibodies, so that these EVs can bind specific antibodies through the z domain on their surface, so as to achieve targeted delivery to specific cells or tissues [1]. The 4z domain, a tetramer of the z domain, has been shown in the literature to be equally capable of constructing EVs displaying antibodies, and has similar binding activity to the z domain.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2299
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 722
  Illegal NgoMIV site found at 962
  Illegal NgoMIV site found at 1130
  Illegal AgeI site found at 517
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 692
  Illegal BsaI.rc site found at 1182
  Illegal SapI.rc site found at 326
  Illegal SapI.rc site found at 1951