Part:BBa_K5246041

C.Crescentus CB2/CB2A hfsE-hfsJ-hfsG - part of the polysaccharide tetrad assembly system

Introduction

Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.

This is a part of the complete holdfast tetrad assembly system BBa_K5246043 used in Vilnius-Lithuania iGEM 2024 project "Synhesion" https://2024.igem.wiki/vilnius-lithuania/. This part can also be used separately for glycolipids consisting of glucose, mannosaminuronic acid, and N-acetyl-D-glucosamine synthesis, but this feature needs more detailed characterization.

Usage and Biology

TBA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 617
  Illegal NotI site found at 3442
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2278
  Illegal BamHI site found at 2426
  Illegal XhoI site found at 2645
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 756
  Illegal NgoMIV site found at 1387
  Illegal NgoMIV site found at 1999
  Illegal NgoMIV site found at 3097
  Illegal AgeI site found at 1086
  
• 1000
  COMPATIBLE WITH RFC[1000]