Part:BBa_K5384002:Design
DDDDK
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The greatest advantage of enterokinase is that when the recognition sequence is fused to the C-terminus of the protein tag, it can be digested without amino acid residue.DDDK enterokinase is a tool enzyme. Enterokinase specifically cleaves peptides or proteins containing recognition sequences and is commonly used in biotechnology to perform specific cleavages of fusion proteins to release the protein of interest. DDDK enterokinase is highly effective and specific. In protein structure and function studies, it is used to accurately cleave the target protein from the fusion protein for subsequent analysis and experimentation. In the biopharmaceutical process, it can be used to prepare protein drugs with specific activities. The enterokinase recognition site on the plasmid serves as a recognition site, allowing the enterokinase to act on the induced proteins.[1,2]
Source
By literature.
References
1.ZHANG Xianghui,TAN Shuhua,LI Taiming. Research progress on the characteristics of enterokinase and its genetic engineering. CNKI, 2005 2.Xu Liang, Wang Wenjing, Zu Xiangyang, Wang Xiaochun. Characteristics of enterokinase digestion of fusion protein Trx-Exendin-4. 《CNKI; WanFang", 2012 3.Cao Zhifei, Li Xinping, Fan Kai, Li Jing, et al. Expression and purification of recombinant bovine enterokinase light chain gene in Pichia pastoris. 《CNKI; WanFang》，2006