Coding
crnA

Part:BBa_K5108004

Designed by: Léa Breton   Group: iGEM24_Toulouse-INSA-UPS   (2024-08-28)
Revision as of 12:54, 29 September 2024 by Yohannquivet (Talk | contribs)

Creatinine amidohydrolase form Pseudomonas putida


    Contents
  1. Usage and Biology
  2. References



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199
    Illegal BsaI.rc site found at 547

Usage and Biology

CrnA is an enzyme that catalyzes the degradation of creatinine into creatine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (crnA) was synthesized in an operon with the gene for creatine degradation. You can find more information about our construct and our results in BBa_K5108009.


References

  1. UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry
  2. Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151

[edit]
Categories
//cds/enzyme
Parameters
biologyPseudomonas putida
proteinCreatinine amidohydrolase
uniprotP83772