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Device
GluColi

Part:BBa_K196014

Designed by: Laetitia Warny   Group: iGEM09_ULB-Brussels   (2009-10-18)
Revision as of 22:00, 21 October 2009 by Aline (Talk | contribs)

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Final GluColi device.

This is the most important biobrick we constructed in order to test the glue production by E. coli. HfsG [1] and hfsH [2] genes come from Caulobacter crescentus. C.crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically. Besides the bacterium is able to synthesize a strong glue. This glue is mainly composed of polysaccharide. Different proteins are required in the holdfast synthesis, export and attachment in C.crescentus (for more details, please refer to the "introduction" section). In our project, we wanted to implement the glue production in Escherichia coli. As E. coli possesses homolog proteins, we decided to only insert the hfsG and hfsH genes in the plasmid. RFP has been added as a reporter gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2263
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1273
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
    Illegal AgeI site found at 1172
    Illegal AgeI site found at 1657
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//function/biosynthesis
//plasmidbackbone/copynumber/high
Parameters
None