Coding

Part:BBa_K187257

Designed by: David Lloyd   Group: iGEM09_Alberta   (2009-10-19)
Revision as of 21:56, 21 October 2009 by JuliaPon (Talk | contribs)

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trpB in pAB

trpB encodes the beta subunit of tryptophan synthase, and is present in the standard biobrick vector pSB1A3

This gene is present in plasmid pAB (part BBa_K187000), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see RFC 47.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 1210
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1210
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 1210
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 1210
    Illegal AgeI site found at 343
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None