Part:BBa_K243029
GSAT Linker
This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. The GSAT linker was created to connect our protein domains Fok_a and Fok_i to a final construct.
Usage and Biology
Normally, Linkers were used to connect two peptides. It is important for that connection that the linker itself has no influence on the connected peptides. Therefore the sequence of the linker is designed with amino acids which do not interact with their environment. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.
The length of the linker is important to guarantee the independent function of two connected parts. If the linker is too short there might be a sterical interference between the parts and if it is too long, it can cause the instability of the construct. Also it is important that the linker has a certain flexibility.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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