Regulatory

Part:BBa_K5332001:Design

Designed by: Qilin Yu   Group: iGEM24_NKU-China   (2024-09-21)
Revision as of 11:12, 29 September 2024 by Ieraser (Talk | contribs) (→‎References)


Reactive oxygen species promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 182
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 182
    Illegal BglII site found at 253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 182
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 182
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing, our primary consideration is whether this part of the selected promoter sequence has the same expression transcriptional effect as the original gene sequence. In addition, some of the selected sequences should still have a high affinity with the oxyR regulator of the upstream ROS response pathway.


Source

From the promoter sequence of katG.

References

[1] Brieger K, Schiavone S, Miller FJ Jr, Krause KH. Reactive oxygen species: from health to disease. Swiss Med Wkly. 2012 Aug 17;142:w13659. doi: 10.4414/smw.2012.13659. PMID: 22903797.

[2] Storz G, Tartaglia LA, Ames BN. The OxyR regulon. Antonie Van Leeuwenhoek. 1990 Oct;58(3):157-61. doi: 10.1007/BF00548927. PMID: 2256675.

[3] Pomposiello PJ, Demple B. Redox-operated genetic switches: the SoxR and OxyR transcription factors. Trends Biotechnol. 2001 Mar;19(3):109-14. doi: 10.1016/s0167-7799(00)01542-0. PMID: 11179804.

[4] Tao K. In vivo oxidation-reduction kinetics of OxyR, the transcriptional activator for an oxidative stress-inducible regulon in Escherichia coli. FEBS Lett. 1999 Aug 20;457(1):90-2. doi: 10.1016/s0014-5793(99)01013-3. PMID: 10486570.

[5] Michán C, Manchado M, Dorado G, Pueyo C. In vivo transcription of the Escherichia coli oxyR regulon as a function of growth phase and in response to oxidative stress. J Bacteriol. 1999 May;181(9):2759-64. doi: 10.1128/JB.181.9.2759-2764.1999. PMID: 10217765; PMCID: PMC93716.