Composite

Part:BBa_K5332000:Design

Designed by: Qilin Yu   Group: iGEM24_NKU-China   (2024-09-29)
Revision as of 11:03, 29 September 2024 by Registry (Talk | contribs)

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ROS promoter->anti-inflammatory factor->adhesion factor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 182
    Illegal PstI site found at 1056
    Illegal PstI site found at 1259
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 182
    Illegal NheI site found at 2030
    Illegal PstI site found at 1056
    Illegal PstI site found at 1259
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 182
    Illegal BglII site found at 253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 182
    Illegal PstI site found at 1056
    Illegal PstI site found at 1259
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 182
    Illegal PstI site found at 1056
    Illegal PstI site found at 1259
    Illegal NgoMIV site found at 400
    Illegal NgoMIV site found at 967
    Illegal NgoMIV site found at 2392
    Illegal NgoMIV site found at 2396
    Illegal AgeI site found at 881
    Illegal AgeI site found at 1982
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Melittin is cytotoxic, and their design can consider reducing cytotoxicity by creating amino acid mutations to alter the sequence. The part currently does not have a designed termination switch for expression and needs further improvement.


Source

Our components are all synthetic, the sequence of Melittin was obtained from NCBI, the adhesion factor CMC was designed and synthesised in-house, and the reactive oxygen species promoter was derived from the KatG gene.

References