Part:BBa_K5332000

ROS promoter->anti-inflammatory factor->adhesion factor

Our part has three special components, the ROS promoter, the anti-inflammatory factor, and the adhesion factor. By virtue of the ROS promoter, it can achieve spatio-temporal specific expression, and its action factors will be expressed only in the environment of high inflammation level; by virtue of the anti-inflammatory factor melittin, it can achieve the reduction of inflammation level in the intestine; by virtue of the adhesion factor, it can be colonised in the intestine and adhere to the beneficial bacteria to improve the microbial environment of the intestinal tract. This plasmid as a whole can be used to alleviate intestinal inflammation, and the promoter portion can be used as a switch for expression in environments with high ROS levels.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 182
  Illegal PstI site found at 1056
  Illegal PstI site found at 1259
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 182
  Illegal NheI site found at 2030
  Illegal PstI site found at 1056
  Illegal PstI site found at 1259
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 182
  Illegal BglII site found at 253
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 182
  Illegal PstI site found at 1056
  Illegal PstI site found at 1259
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 182
  Illegal PstI site found at 1056
  Illegal PstI site found at 1259
  Illegal NgoMIV site found at 400
  Illegal NgoMIV site found at 967
  Illegal NgoMIV site found at 2392
  Illegal NgoMIV site found at 2396
  Illegal AgeI site found at 881
  Illegal AgeI site found at 1982
  
• 1000
  COMPATIBLE WITH RFC[1000]