Composite

Part:BBa_K243026

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-18)
Revision as of 21:47, 21 October 2009 by Isabel (Talk | contribs)

Long Linker-Fok_a

This part is the combination of the parts Long Linker ((GlySerGlyGly)x3) and the protein domain Fok_a.

Usage and Biology

This combination allows a direct coupling of the protein domain to another part, e.g. a tag for purification. The linker can secure an independent function of the protein domain when it is fused to another part. We used the Long Linker to avoid sterical interferences between our protein domain Fok_a and the other connected part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 529


[edit]
Categories
Parameters
n/apMA Long Linker-Fok_a