Coding

Part:BBa_K5387000:Design

Designed by: iGEM24_Linkoping   Group: iGEM24_Linkoping   (2024-08-20)
Revision as of 10:31, 29 September 2024 by Feliciawedlund (Talk | contribs)

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Design Notes

The sequence has been optimized for expression in E. coli.

The vector used was pET-28a(+), which His-ag we utilized when expressing our protein.

Primers for cloning into pET-28a(+) between restriction sites NdeI and EcoRI:

fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'