Composite
Part:BBa_K5507010
Designed by: josiazhang josiazhang Group: iGEM24_HW-ShanghaiUnited (2024-09-29)
gdh-5-Ptac-kana-rrnB-T-gdh-3
For the knockout of the gdh protein in K. xylinus. The Ptac-kana-rrnB-T construct serves as a marker gene in the genome to indicate whether gene recombination has been successful. gdh-5 and gdh-3 are the flanking sequences of the gdh gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1886
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Categories
Parameters
None |