Composite

Part:BBa_K5507010

Designed by: josiazhang josiazhang   Group: iGEM24_HW-ShanghaiUnited   (2024-09-29)
Revision as of 09:55, 29 September 2024 by Registry (Talk | contribs)

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gdh-5-Ptac-kana-rrnB-T-gdh-3

For the knockout of the gdh protein in K. xylinus. The Ptac-kana-rrnB-T construct serves as a marker gene in the genome to indicate whether gene recombination has been successful. gdh-5 and gdh-3 are the flanking sequences of the gdh gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1886


[edit]
Categories
Parameters
None