Composite
Part:BBa_K180015
Designed by: Richard Wilson, Stephanie Chambers Group: iGEM09_Southampton (2009-10-19)
Paper - Primary plasmid (part 2) [tac pR, GFP and RhlI generator]
Part of the iGEM2009 Team:Southampton rock-paper-scissors 'paper' primary plasmid.
GFP and RhlI are generated under tac pR regulation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2117
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
control | trp, lac |
direction | forward |
emission | 511 nm |
emit | 511 |
excitation | 501 nm |
excite | 501 |
lum | |
n/a | Paper - Primary plasmid (part 2) [tac pR, GFP and RhlI generator] |
protein | GFPmut3b |